Expression profiling of Echinococcus multilocularis miRNAs throughout metacestode development in vitro
Fig 1
Echinococcus multilocularis samples analyzed in this work and their relationship with the stages of the parasite’s life cycle in vivo.
MCvivo: metacestodes extracted from experimentally infected jirds, MCvitro: metacestodes cultured in vitro in aerobic conditions, MCana: metacestodes cultured in vitro in axenic and anaerobic conditions, PC1: primary cells cultured in vitro for 48 hs, PC2: primary cells cultured in vitro for 7 days containing central cavities and PC3: primary cells cultured in vitro for 21 days containing several central cavities and released mini vesicles (metacestodes), naPS: non-activated protoscoleces, aPS: in vitro activated protoscoleces by pepsin/low pH treatment. Echinococcus multilocularis samples analyzed in this work (A). Relationship between Echinococcus multilocularis samples analyzed in this work and the stages of Echinococcus multilocularis life cycle in vivo (B).