Aedes aegypti post-emergence transcriptome: Unveiling the molecular basis for the hematophagic and gonotrophic capacitation
Fig 5
Increased expression of genes involved in mitochondrial processes drive respiratory rates and OXPHOS.
Heatmap of genes related to pyruvate and proline oxidation (A), the y-axis shows gene codes and x-axis shows female (F) and male (M) time courses from 2 to 96 hours, details in S13A Fig; typical traces of oxygen consumption rates (OCR) (B), the OCR (red line) and concentration (blue line) were obtained from mechanically permeabilized Aedes Rio female heads at 24 hours PE using Pyr+Pro as substrates; oxygen consumption linked to ATP synthesis (OXPHOS) (C); maximum respiratory rates (ETS) (D); Cytochrome c oxidase (COX) activity (E); Leak represents the oxygen consumption in the presence of high substrate concentration but in the absence of ADP (F); residual oxygen consumption (ROX) (G); citrate synthase activity (H). Bar graphs show mean (SEM) for male and female at different PE time points. Interaction p-value between sex and time factors (two-way ANOVA) are displayed above the bars. ns = not significant; *p-value <0.05; **p-value <0.01; ***p-value <0.001.