Characterization of flavivirus infection in salivary gland cultures from male Ixodes scapularis ticks
Fig 2
LGTV titer from infected male SG cultures.
Infectious virus release from Langat virus (LGTV)-infected male I. scapularis salivary glands (SGs) was quantified using immunofocus assays. Experimental and control samples were inoculated with 5 x 104 (A), 5 x 105 (B), and 5 x 106 (C) focus-forming units of LGTV. Immunofocus assays were performed using supernatants collected from experimental wells with SGs infected with LGTV and control wells with LGTV inoculum and no SGs. Error bars represent the standard error of the means. Viral titers per timepoint were averaged and the log10 values were calculated. Initial inoculum (control) logarithmic values were then subtracted from the corresponding infectious virus release logarithmic values per timepoint to visualize timepoints of peak virus release (D-F). These data are representative of 2–3 biological replicates and 2 technical replicates per timepoint. Data were processed using GraphPad Prism software v7.04.