ISG15 overexpression compensates the defect of Crimean-Congo hemorrhagic fever virus polymerase bearing a protease-inactive ovarian tumor domain
Fig 2
Levels of structural proteins and minigenome RNA.
Cells were transfected as indicated for Fig 1. (a) and (b) Immunoblot analysis of lysates from tc-VLP donor cells. (a) Representative blots. (b) Quantification of immunoblot signals obtained by calculating the ratio of the respective L signal to tubulin and setting wt L to 100% (n = 3). (c) Immunoblot analysis of lysates from tc-VLP donor cells (top panels) and supernatants derived from them (bottom panels, representative blots shown). (d) Quantification of immunoblots as shown in (c) (n = 3). (e) and (f) Minigenome RNA synthesis. Total RNA from donor cells (e) and VLP-containing cell supernatants (f) were tested by negative-strand RNA-specific RT-qPCR for the Ren-Luc sequence. For each experiment, mean values and the standard deviations of 3 independent repeats are shown. Two-tailed, paired Student’s t test without (b, d) or with Bonferroni correction for multiple comparisons (e, f) were used. *, P < 0.05; n.s., not significant; n.t. non-transfected; CTRL, empty vector.