De novo identification of toxicants that cause irreparable damage to parasitic nematode intestinal cells
Fig 5
Larvae ensheathed with deformation (LED) responses to varying Staurosporine timing and Sunitinib concentrations.
(A) The observed phenotypes are quantified after 1 μL of 25 μM Staurosporine was delivered to A. suum L3 (from 8-day rabbit infections) at time 0 (Day 0) and after 2 days in culture (Day 2), compared to control larvae. P values indicate the results of a two-tailed T-test with unequal variance, compared to control. (B) A. suum treated at Day 2 demonstrate the non-motile and LED phenotypes, 5 days following treatment. The second panel is magnified to highlight the anterior and posterior damage to the worm. (C) A. suum treated at Day 0 are immotile and do not have a visible LED phenotype 5 days after treatment. (D) Untreated controls are motile and have molted to L4. (E) Increasing concentrations of Sunitinib (applied to Day 0 L3 larvae for 5 days) result in a lower occurrence of the LED phenotype. A one-way analysis of variance (ANOVA) indicated significant variation among conditions (P = 1.7x10-5). Samples grouped significantly into groups a and b (as indicated) according to Tukey HSD post-hoc test.