An investigation into the role of chronic Schistosoma mansoni infection on Human Papillomavirus (HPV) vaccine induced protective responses
Fig 6
Production of IL-4 cytokine in supernatants during Specific (HPV, SEA and SWAP) and non-specific stimulation (CONA) and absence of stimulant (MED) in Schisto-infected+HPV, Schisto/PZQ+HPV vaccine and HPV-only (Control) groups. PBMCs collected from the 3 groups (n = 3) at the 4 time points; Week 0 (pre-vaccination), Week 4 (4 weeks after the 1st vaccination and before the booster), Week 6 (6 weeks after the 1st vaccination and 2 weeks after the 2nd dose), Week 8 (8 weeks after 1st dose and 4 weeks after 2nd dose). These PBMCs were stimulated with respective Antigens (HPV, SEA, SWAP and CONA) for 48h, after which the supernatants were analyzed using ELISA. The bars represent the mean IL-4 levels of each group at each time point. Non-parametric analysis (Kruskal Wallis, Dunn’s post hoc analysis) was applied. Data was considered statistically significant if p≤0.05.