Intestinal UDP-glucuronosyltransferase as a potential target for the treatment and prevention of lymphatic filariasis
Fig 4
Bm-UGT siRNA incubation reduces target transcript and protein levels in adult B. malayi worms.
Adult female worms were incubated with Bm-UGT siRNA, scrambled siRNA, or media alone. (A) Target mRNA levels (n = 3) in the specific siRNA and scrambled siRNA treated groups were measured by RT-qPCR relative to the media control normalized to Bm-gapdh, (Day 1, p = 0.0002; Day 3, p<0.0001; Day 6, p = 0.0034). p values were generated using an ordinary one-way ANOVA followed by Tukey’s multiple comparison test. These experiments were successfully repeated twice and the data shown is from a single representative experiment; mean ± SEM. (B) Bm-UGT expression (n = 5) was evaluated 24 hrs post-siRNA incubation using anti-Bm-UGT peptide antibodies. (C) Western blot quantification was performed using the ImageStudioLite software. Depicted are the signal intensities of anti-UGT normalized to beta-actin.