CXCR3 chemokine receptor guides Trypanosoma cruzi-specific T-cells triggered by DNA/adenovirus ASP2 vaccine to heart tissue after challenge
Fig 5
CXCR3 receptor is important to cytotoxicity of specific CD8+ T cells during the immunization.
a-Histograms represent the frequency of CFSElow and CFSEhigh population that were transferred to βgal, ASP2, and ASP2+αCXCR3 groups. After 12 hours of transference, the percentage of CFSEhigh cell lyses was calculated as described in the methods section. b-Cytotoxicity percentage of specific CD8+ T cells during immunization and treatment with anti-CXCR3. c-Percentage of cytotoxicity of specific CD8+ T cells during immunization, infection and treatment with anti-CXCR3 d-Granzyme B MFI on specific CD8+ T cells from βgal+T.cruzi, ASP2+T.cruzi, and ASP2+αCXCR3+T.cruzi groups. Results are shown as individual values and as the mean ± SEM for each group (n = 3). One of three independent experiments is presented. Statistical analysis was performed using the One-Way ANOVA and Tukey’s HSD tests. Symbols indicate that the values observed were significantly different between the groups (*p < .0001; **p < .01; #p < .0001; ζp = 0.0001) and n.s means no significant.