The cell fate regulator NUPR1 is induced by Mycobacterium leprae via type I interferon in human leprosy
Fig 4
M. leprae induction of NUPR1 is dependent on type I IFN signaling.
(A). Effect of different doses of IFN-β on the induction of NUPR1 gene expression in MDMs measured by qPCR (n = 4). (B). Effect of IFNAR blocking with different doses of IFN-β on NUPR1 gene expression in MDMs measured by qPCR (n = 3). (C) and (D). Evaluation of the effect of M. leprae infection on NUPR1 gene expression during blockage of IFN-β signaling at 24 and 48h measured by qPCR (n = 6). Statistical analyses were performed using repeated measures one way-ANOVA (A), repeated measures two way-ANOVA with the Geisser-Greenhouse correction (B) and repeated measures two way-ANOVA (C and D) followed by Bonferroni’s multiple comparisons test (A-D).