The effect of amantadine on an ion channel protein from Chikungunya virus
Fig 4
Mammalian cell expression of EGFP-6K shows predominant ER expression: Confocal microscopy images of HEK293T cells expressing EGFP-6K alone or in conjunction with myc-E1 and/or myc-E2 glycoproteins.
Panels (A-E) show images of cells expressing EGFP-6K and stained with fluorescent markers corresponding to ER (A, anti-KDEL, red), golgi (B, GM-130, red), plasma membrane (C, red, WGA), nucleus (D, DAPI, blue) and mitochondria (E, mitotracker, red). Panels (F) and (G) contain cells expressing EGFP-6K along with E1(Panel F) and E2 (Panel G) respectively. Panel (H) contain cells co-expressing EGFP-6K and E2 and stained with WGA (red). Scale bars: (A-D, G: 10 μm); (E, F, H: 5 μm).