The effect of amantadine on an ion channel protein from Chikungunya virus
Fig 1
6K sequence analysis and GST-6K purification: (A) A comparative analysis of the hydrophobicity of 6K peptides encoded by different members of the alphavirus family. Kyte-Doolittle hydrophobicity plots and multiple sequence alignment of 6K peptides from different alphaviruses—Chikungunya (CHIKV), Semliki Forest Virus (SFV), Sindbis Virus (SINV), Ross River Virus (RRV) and Mayaro Virus (MV) are shown. Sequence alignment color legend: red-identical residues; blue-highly similar residues and green-less similar residues. (B) Potential transmembrane domains in CHIKV 6K as predicted by different servers as indicated. (C) SDS-PAGE of GST-6K after pulldown with GST beads. Lane 1 corresponds to the molecular weight ladder, while lanes 2 to 9 correspond to protein fractions. (D) Gel filtration profile of GST-6K on Superdex 200 10/300 G/L column.