Distinct monocyte subset phenotypes in patients with different clinical forms of chronic Chagas disease and seronegative dilated cardiomyopathy
Fig 2
Phenotyping of the different monocyte subsets.
PBMCs were stained for CD14, CD16, CCR2, HLA-DR and CD45RA and analyzed by flow cytometry. Monocytes were gated by forward and side scattering. Each point represents the MFI for CD45RA (A) and CCR2 (B) in the different monocytes subsets among T.cruzi-infected subjects (G0 n = 21; G1-G2-G3 n = 7), seronegative dilated cardiomyopathy patients (DCM) (n = 5) and uninfected healthy controls (n = 6). Black diamonds indicate G1 benznidazole-treated patients; the black circle indicates a G2 benznidazole-treated patient. The median values are indicated by the horizontal lines. * P < 0.05; Differences among groups were evaluated by ANOVA, the Kruskal-Wallis test followed by post-tests or a t-test.