Sensitive and specific detection of Crimean-Congo Hemorrhagic Fever Virus (CCHFV)—Specific IgM and IgG antibodies in human sera using recombinant CCHFV nucleoprotein as antigen in μ-capture and IgG immune complex (IC) ELISA tests
Fig 3
Analysis of paired CCHF patient samples: Raw data (OD420 – OD620).
A serum panel consisting of 30 paired serum samples from 15 CCHF patients, serum samples from 12 CCHF patients collected approximately one year after recovery from CCHFV infection, a set of a priori CCHFV IgM/IgG negative serum samples (neg) ((A), (B): n = 120; (C), (D): n = 15, see S1 Fig), and 98 CCHFV IgM/IgG IIFT negative sera from healthy blood donors from Kosovo (HD K) were analyzed with the BLACKBOX CCHFV IgM ELISA (A), the BLACKBOX CCHFV IgG ELISA (B), the VectoCrimean-CHF-IgM ELISA (C) and the VectoCrimean-CHF-IgG ELISA (D). Cut-off values (represented by dotted lines) were determined by ROC analysis ((A): 0.129, (B): 0.161) or according to the manufacturer’s instructions ((C): 0.240, (D): 0.246), respectively (see S2 Fig). Filled circles indicate PCR positive serum samples.