Analytical sensitivity and specificity of a loop-mediated isothermal amplification (LAMP) kit prototype for detection of Trypanosoma cruzi DNA in human blood samples
Fig 3
Analytical sensitivity of LAMP assay.
Top panel: Fluorescence observed with the naked eye from serial dilutions obtained from 3 different aliquots of DNA from CL Brener stock (DTU VI). Bottom panel: Fluorescence observed with the naked eye from serial dilutions obtained from 3 different aliquots of DNA from Sylvio X10 stock.The aliquots were expressed in fg/μL. A: 0; B: 1.0 x 10−3; C: 1.0 x 10−2; D: 1.0 x 10−1, E: 1. NC: Non template control.