Identification of three immunodominant motifs with atypical isotype profile scattered over the Onchocerca volvulus proteome
Fig 1
Peptide array analysis of serum samples from O. volvulus infected individuals.
(A) Volcano plot showing the log2 fold change in the x-axis and the–log10 adj. p-value in the y-axis for every single peptide analyzed in the arrays. Peptides indicated in blue represent the set of 1110 immunoreactive peptides; peptides indicated in green represent the set of 249 immunodominant peptides. (B) Overview of peptide array design and strategy for selection of candidate immunodominant peptides. (C) Summary table of the performance of the 1110 immunoreactive peptides. The response for every peptide for the different samples was compared to the preset thresholds (signal >4842 scored as confirmed positive for patients, signal < 837 scored as confirmed negative for healthy controls). These thresholds were based on the signals of the healthy control sample set on the 1110 immunoreactive peptides (as described in Fig 1B).