Analgesic Effect of Photobiomodulation on Bothrops Moojeni Venom-Induced Hyperalgesia: A Mechanism Dependent on Neuronal Inhibition, Cytokines and Kinin Receptors Modulation
Fig 1
Effect of photobiomodulation on mechanical allodynia (A) or hyperalgesia (B and C) of mice.
Animals were injected intraplantar with Bmv (1 μg; ■) and after 30 min and 3 h were treated with LLL at 685 nm (Bmv + LLLT; ●) or a combination of laser and antivenom (Bmv + LLLT + AV; ▼). Allodynia was measured by the mechanical response to tactile stimulation assessed by a von Frey filament of 0.407 g (3.61 filament; A). Hyperalgesia was measured by the mechanical response to nociceptive stimulation assessed by von Frey filaments of 0.692 g (3.84 filament; B) and 1.202 g (4.08 filament; C). Pain threshold was determined before (baseline values, time 0) and 1, 3, 6, 12 and 24 h after BmV injection. Animals injected only with Bmv or with a combination of Bmv+AV (□) were submitted to the same protocol. Each point represents the mean ± SEM of five to seven animals per group. (*p<0.05, ≈ p<0.01 or #p<0.001 vs Bmv group are indicated).