Melioidosis: Clinical impact and public health threat in the tropics
Fig 3
Ultrastructural examination of B. pseudomallei used for infection.
(A) Scanning electron microscopy (SEM) image showing Bp before the infection with U-937 cells. (B) Bp-infected cells were examined by a transmission electron microscopy (TEM) with large number of bacilli (Bp) presented in the cytoplasm. Light micrograph showing B. pseudomallei infection of mouse spleen; section stained by Haematoxylin and Eosin (H&E) imaged with different magnification. (C–D) Large abscesses (Abs) with focal areas of necrosis, surrounded by a rim of meshed fibrous tissue (f) are evident after 2 weeks IP challenge with 1.7x105 CFU/ml. The bacterial invasion is more in spleen and liver than kidney. Bp, B. pseudomallei; abs, abscess; f, fibrous tissue; rs, rough surface; s, septa; sb, single bacilli; n, nucleus; ph, phagocytosis.