Delayed Time-to-Treatment of an Antisense Morpholino Oligomer Is Effective against Lethal Marburg Virus Infection in Cynomolgus Macaques
Fig 2
Viral titers and genome equivalents in serum.
Mean serum (A) infectious virus and (B) viral RNA concentration in each treatment group. Infectious virus was determined by plaque assay with a lower limit of quantitation of 1,000 PFU/mL, and viral RNA was determined by quantitative RT-PCR with a lower limit of quantitation of 1.33 x 105 genome equivalents/mL. Serum viral RNA on day 8 with the peak serum infectious virus for each animal and the mean and standard deviation indicated by horizontal line and error bar. ANOVA P < 0.01 and Tukey’s multiple comparisons significant differences are indicated by (**) for P = 0.001. (C) Area under the serum viremia versus time curve on the ordinate versus time to treatment initiation on the abscissa. The blue open circles represent viral RNA/mL and the filled green circles represent PFU. The slope of the genome equivalents/mL is 0.0154 ± 0.00104 and r2 of 0.991, P = 0.045. The slope of the PFU/mL is 0.0172 ± 0.006, r2 of 0.782, P = 0.116. Values <LLOQ were converted to LLOQ for display and analysis.