Anopheles Midgut Epithelium Evades Human Complement Activity by Capturing Factor H from the Blood Meal
Fig 8
Confocal microscopy images showing colocalization of bound human FH with the glycocalyx layer at the apical surface of the proventriculus and the anterior midgut epithelium of A. stephensi mosquitoes.
(A, D and G) FH binding to the surface of mosquito the proventriculus and the anterior midgut epithelium detected by goat Anti-FH. (B, E and H) Binding of ConA-Alexa 594 to the glycocalyx at the apical surface of the proventriculus and the anterior midgut epithelium. (C, F and I) Overlay images showing colocalization bound FH with the glycocalyx layer defined by bound ConA-Alexa 594. FH was found to colocalize with approximately one quarter of the luminal side of the glycocalyx layer (I). FH binding also formed a thin layer lining the luminal surface of the glycocalyx (C, F and I). The white, yellow and red scale bars are 50, 5 and 1 μm, respectively. G, H and I are digitally enlarged cropped images of the selected areas on D, E and F, respectively.