Use of Humanised Rat Basophilic Leukaemia Cell Line RS-ATL8 for the Assessment of Allergenicity of Schistosoma mansoni Proteins
Figure 6
Luminescence data of RS-ATL8 assay demonstrating the allergenicity of SmTAL-1.
Recombinant SmTAL1 and SmTAL2 were expressed in E. coli (rTAL1, rTAL2; both 10 ng/ml) and in WGL (WG TAL1, WG TAL2; diluted 1∶104). Cells were sensitized for 16 hours with pooled, virally inactivated plasma from individuals living in a S. mansoni endemic area in Uganda (diluted 1∶100) and stimulated with the recombinant antigens. The experiment included the negative controls: unsensitized and unstimulated cells (medium), cells incubated only with treated plasma diluted 1∶100 v/v (serum) and the positive control (IgE+aIgE, both 1 µg/mL). Data are mean +SD of the readings of three separate wells. Multiplicity adjusted p-values were obtained by ANOVA followed by Dunnett's test for each condition compared with plasma only control. ****: p<0.0001; ***: p<0.001; n.s.: not significant.