Analysis of Epitopes on Dengue Virus Envelope Protein Recognized by Monoclonal Antibodies and Polyclonal Human Sera by a High Throughput Assay
Figure 3
Binding specificity and predominant epitope recognized by anti-E Abs in serum from a DENV1 case.
(A) Binding specificity was examined by Western blot analysis as described in Methods. Lysates of 293T cells transfected with pCB-D1 (D1 tr) were also included. (B) Dot blot assay presented as in Figure 1A and 1C to 1E (except that WT dot in row 8C and 153NA dot in row 2H were omitted) was probed with the tested serum or mixed sera, which consisted of a pool of 9 sera from confirmed dengue patients [44]. The relative intensities of two-fold dilutions of WT dots in row 1 were presented as in Figure 1D. R.I. of each mutant was shown as in Figure 1E. One representative experiment of two was shown. (C) Capture ELISA using WT or mutant VLPs was presented as in Figure 1F. Upper graph in panel C shows comparable amounts of WT and mutant VLPs added.