The Heme Biosynthetic Pathway of the Obligate Wolbachia Endosymbiont of Brugia malayi as a Potential Anti-filarial Drug Target
Figure 3
Effects of succinyl acetone (SA) and N-methyl-mesoporphyrin (NMMP) on the motility of B. malayi adult worms ex vivo and C. elegans larval growth.
A) Effect of SA on B. malayi adult males, B) Effect of SA on B. malayi adult females, C) Effect of NMMP on B. malayi adult males, D) Effect of NMMP on B. malayi adult females, E) Effects of SA and NMMP on growth of C. elegans. The scale of viability (A–D) is arbitrarily measured by the relative motility of the adult worms: 0, no motility; 1, slight movement clearly observed under microscope; 2, minor movement readily observed by eye; 3, non-continual moderate movement; 4, continual moderate movement; 5, continual active movement. Control B. malayi worms (C, D) were cultured in medium containing 1% ethanol and 0.0004 N NaOH (“solvent only”) with and without 100 µM hemin. Data is compiled from 3 independent experiments (see Materials and Methods). Control C. elegans worms (E) were grown in 1% ethanol with 0.0004 N NaOH, which corresponds to the concentration of ethanol and NaOH present in the 100 µM NMMP test.