Mechanisms of GII.4 Norovirus Persistence in Human Populations
Figure 2
Phylogenic Reconstruction of the GII.4 Capsid Sequence
A multiple alignment was generated using 176 full length capsid amino acid sequences and trees were generated using BI and MP analysis. The clusters are marked as follows: yellow, sequences from Camberwell cluster; red, sequences from Grimsby cluster; blue, sequences from the Farmington Hills cluster; green, sequences from the Hunter cluster; orange, sequences from the Sakai cluster; and purple, sequences from the Den Haag cluster. The trees are drawn to similar scales and are rooted with the earliest Camberwell cluster.
(A) BI predicts that Camberwell gave rise to Grimsby, which gave rise to an LCA from which Farmington Hills and a LCA for the three extant clusters evolved independently (for full tree see Figure S2).
(B) The MP bootstrapped tree generated by MEGA 4.0 was similar to the Bayesian tree with the exception that the Grimsby cluster gave rise to the Farmington Hills cluster, and a LCA of all extant clusters arose from Farmington Hills (for full tree see Figure S3). A MP tree generated by PAUP 4.0b10 predicted similar results (Figure S4).