Tumor-associated macrophage subtypes on cancer immunity along with prognostic analysis and SPP1-mediated interactions between tumor cells and macrophages
Fig 2
The strongest communication between different subgroups is through the SPP1 pathway.
(A) Pie chart showing the percentage distribution of different signaling pathways. (B) Line graph showing the intercellular action of the SPP1 pathway. (C) Contribution of different receptors of SPP1 action. (D) SPP1 action with receptor CD44, ITGA8 and ITGB1, ITGA4 and ITGB1, ITGA5 and ITGB1, ITGAV and ITGB5. (E) Expression of different receptors in different subpopulations. (F) Human SPP1 levels in M0, tumor cells and TAM were detected by ELISA. (G) Human TGF β, IL10 and VEGF levels in MDA-MB 231 (231)-induced TAM treated by rh SPP1 protein and anti-SPP1 antibody were detected by ELISA. Data were shown as mean ± SD and are representative of three independent experiments. P values were calculated using the 2-tailed 2-sample t test. *P < 0.05, **P < 0.01, ***P < 0.001.