A dynamic population of prophase CENP-C is required for meiotic chromosome segregation
Fig 2
CENP-C is exchanged at the centromeres during meiotic prophase.
Immunocytology was performed on oocytes ubiquitously expressing GFP-CENP-C (green), plus or minus GL00409 (Cenp-CRNAi), and HA-CENP-C (red) using the mata promoter. All scale bars represent 5 μm and DNA is blue. A) GL00409 recognizes a sequence in the 5’ UTR, and, therefore, degrades GFP-Cenp-C but not HA-Cenp-C. Images show stages 1, 5 and 14 with (B) expression of GFP-CENP-C and HA-CENP-C, (C) expression of GFP-CENP-C, Cenp-CRNAi, and HA-CENP-C, and (D) expression of GFP-CENP-C with Cenp-CRNAi. E) The relative intensity of GFP-CENP-C was measured at each stage in each genotype (n = 70, 117, 28, 23; 12, 19, 104, 156; 50) with error bars showing standard deviation. For each genotype, the intensity between stage 1 and stage 14 was compared using an unpaired t-test (***p = 0.001; ****p<0.0001).