A negative feedback loop is critical for recovery of RpoS after stress in Escherichia coli
Fig 5
Rapid RpoS recovery from phosphate and glucose starvation requires Crl.
A) RpoS degradation during recovery from phosphate starvation in the WT, Δcrl (SB147) and crl-R51A (SB148) strains (quantitation from Western Blot as shown in S5A Fig). Experiment was performed as described in Fig 1A. B) RpoS degradation during recovery from glucose starvation in the WT and Δcrl (SB147) strains (quantitation from Western Blot as shown in S5B Fig). Experiment was performed as described in Fig 1A. C) RpoS and RpoS-Lac accumulation experiments (no chloramphenicol added) during recovery from phosphate starvation in strains SG30013 (crl+) and SB180 (Δcrl), both containing the RpoS-Lac fusion; quantitation is from Western Blot as shown in S6C Fig. D) RpoS and RpoS-Lac accumulation experiments (no chloramphenicol added) during recovery from phosphate starvation in WT (crl+; circles) and Δcrl (triangles) strains containing the RpoS-Lac fusion but lacking rpoS (INH28 (crl+) and SB176 (Δcrl); quantitation is from Western Blot as shown in S6C Fig. E) RpoS and RpoS-Lac accumulation experiments during recovery from phosphate starvation in strains lacking rpoS and carrying rssA2::cm, which overproduces RssB, SB150 (crl+) and SB174 (Δcrl), both containing the RpoS-Lac; quantitation is from Western Blot as shown in S6C Fig.