The circadian clock gene bmal1 is necessary for co-ordinated circatidal rhythms in the marine isopod Eurydice pulchra (Leach)
Fig 4
Circatidal locomotor rhythms in knockdown animals in season 2 (2022).
As in Fig 3, with locomotor swimming events monitored under constant conditions. Four collections from (A) early summer, new moon (B) mid-summer, full moon(C) late summer, new moon (D) autumn, two collections, full and new moon. In D, animals were maintained in LL after injection. E-K Statistical analyses of time series in A-D (see S1 Table for all ANOVA results). E. Modulation index. F. Amplitude of subjective day and night locomotor components combined (log10 units) G. Night component only H. Day component only I. circatidal period from spectral analysis J. circatidal spectral power K. proportion of arrhythmic animals in each collection for each Knockdown where collection colours correspond to the high tide arrow colours in A-D. * p<0.05, **p<0.01 (from ANOVAs and post hoc tests, see text). Means shown as horizontal black lines. Ns for WTYFPi, Epbmal1i and Epcry2i A; 22 and 18 (no Epcry2i animals), B; 22, 17, 10 C; 30, 26, 20 and D; 20, 27 (no Epcry2i), respectively. X-axis, circadian time (CT, h) in constant conditions. Y-axis, locomotor (swimming) events per 30 min time bin.