The circadian clock gene bmal1 is necessary for co-ordinated circatidal rhythms in the marine isopod Eurydice pulchra (Leach)
Fig 3
Circatidal rhythms in knockdown animals in season 1 (2016).
A-C. Mean activity in 30 min bins of animals collected during spring tides in early summer, new moon (A), midsummer full moon (B) and in late summer/early autumn full/new moons (C). Standard errors of means are omitted for clarity. Panels on left are WTYFPi (green) middle are Epbma1i (red) and right are Epcry2i (blue). Vertical arrows represent high tides at Llanddona beach. Animals were collected on the beach, injected with dsRNAi constructs the following day and left for 3 days in DD before being placed in activity monitors to acclimatise for one further day in DD. Activity recording therefore, started at CT96. D-J Statistical analyses of time series in A-C (see S1 Table for all ANOVA results). D. Modulation index E. Overall locomotor amplitude (subjective day plus night locomotor components) in log10 units. F. Night component only G. Day component only H. circatidal period from spectral analysis I. circatidal spectral power J. proportion of arrhythmic animals in each collection for each Knockdown- collection colours correspond to the high tide arrow colours in A-D. * p<0.05, **p<0.01 from two-way ANOVAs and Dunnett’s post hoc tests, * from Tukey post hoc (see Table 1 and S1 Table). +p<0.05 ++ p<0.01 from one-way pooled seasonal ANOVA. Means shown as horizontal black lines. N for WTYFPi, Epbmal1i and Epcry2i are in A, 23, 22, 20, B, 20, 22, 20 and C, 27, 57, 29, respectively. Y-axis, locomotor (swimming) events per 30 min time bin. X-axis Circadian Time (CT, h) in constant DD conditions.