RBMX involves in telomere stability maintenance by regulating TERRA expression
Fig 4
RBMX regulates TERRA level by interacting with ZCCHC8.
(A) Pair-wise Pearson correlation between RBMX and ZCCHC8 mRNA levels. The website tool GEPIA was used for correlation analysis, with the gene expression data (TPM value) from TCGA tumor (n>1000). (B-G) RBMX binds to ZCCHC8 and TERRA in 293T cells. (B) Schematic diagram. Interaction between ectopically expressed GFP-tagged RBMX, endogenous ZCCHC8 and TERRA was detected by co-immunoprecipitation with anti-GFP antibody in RNase-free condition. Half of the co-IP eluates were used for protein detection by western blot (C), while the other half were used for TERRA detection by RT-qPCR, including TERRA transcribed from 6q, 10q, 12p, and 19p chromosomes (D-G). (H) TERRA increases in U2OS cells transfected with indicated siRNAs. Slot blot was performed to determine the TERRA level, with 18S RNA as loading control. Error bars, standard deviations from ≥3 biological replicates. p values, two-tailed Student’s t test (****P <0.0001).