Blocking Caspase-1/Gsdmd and Caspase-3/-8/Gsdme pyroptotic pathways rescues silicosis in mice
Fig 7
Inactivation of Gsdmd and Gsdme by dimethyl fumarate rescue mouse silicosis.
a The activation of Gsdmd, Gsdme and Caspase-1 was measured through immunoblot analysis, both in WT and Gsdmd-/- BMDMs. b, c The release of IL-1β and IL-18 in WT and Gsdmd-/- BMDMs culture medium. d Immunofluorescence images of macrophages (CD11b+F480+), neutrophils (CD11b+Ly6G+) and monocytes (CD11b+Ly6C+) in lung tissues of mice. Arrowheads indicate the infiltrated immune cells that labelled with antibodies against CD11b (green), F4/80 (red) and Ly6G/6C (red). DAPI (grey) localizes with the nuclei. The scale bar represents 50 μm. e The levels of IL-1β and IL-18 in BALF of mice, n = 3. f H&E (upper) and Masson (lower) staining of the indicated mouse lung sections 14 days after the initial silica challenge. The scale bar represents 100 μm. g The activation of Gsdmd, Gsdme and Caspase-1 was measured through immunoblot analysis. Results are expressed as mean ± SD from three independent experiments. NS, not significant; **P<0.01 and ***P<0.001.