P450 gene duplication and divergence led to the evolution of dual novel functions and insecticide cross-resistance in the brown planthopper Nilaparvata lugens
Fig 5
Sensitivity of transgenic strains of Drosophila melanogaster expressing mutated CYP6ER1 variants to imidacloprid.
Lethal Concentration 50% (LC50) values derived from full dose-response bioassays are displayed for D. melanogaster strains expressing CYP6ER1vL variants to which mutations found in CYP6ER1vA were added, or CYP6ER1vA to which mutations were removed. For comparison LC50 values derived from flies expressing the wildtype versions of CYP6ER1vA and CYP6ER1vL are shown. Error bars show 95% CI (n = 4). Significant differences (p = < 0.05) in LC50 values of fly lines expressing CYP6ER1 mutated variants and the respective wildtype fly line (CYP6ER1vA or CYP6ER1vL), identified by z-tests using the compParm() function of the ’drc’ package [36], are indicated using an asterisk above relevant bars. See also S7 Table.