P450 gene duplication and divergence led to the evolution of dual novel functions and insecticide cross-resistance in the brown planthopper Nilaparvata lugens
Fig 2
Sensitivity of transgenic strains of Drosophila melanogaster expressing different CYP6ER1 variants to three insecticides.
Lethal Concentration 50% (LC50) values for each strain derived from full dose-response bioassays are displayed for A) ethiprole, B) fipronil and C) imidacloprid. Data for D. melanogaster strains expressing CYP6ER1 variants observed in field populations of N. lugens (CYP6ER1vA, B, C, F) and the lab susceptible strain NLS (CYP6ER1vL) is shown. For comparison the sensitivity of a control fly line (control) of the same genetic background but without a transgene is shown. Error bars show 95% CI (n = 4). Significant differences (p = < 0.05) in LC50 values of fly lines expressing CYP6ER1 variants and the fly line without a transgene, identified by z-tests using the compParm() function of the ’drc’ package [36], are indicated using an asterisk above relevant bars. See also S1–S3 Tables.