The essential Rhodobacter sphaeroides CenKR two-component system regulates cell division and envelope biosynthesis
Fig 4
Identification of the CenR DNA binding motif.
(A) WebLogo [134] representation of CenR consensus sequence determined using the MEME motif finder. Of the 59 promoters identified in ChIP-seq experiments, 31 operons contained the putative CenR consensus sequence TGA-(N8)-TGA. (B) Location and orientation of the 31 identified CenR binding sites, in respect to the previously determined TSS [41] and promoter -35 and -10 elements [126] for directly activated (green) and repressed (red) transcription units. The DNA sequence of each binding motif is listed in Table 1. (C) Histogram of the distance from the center of each putative CenR binding site and the TSS of the respective transcription unit mapped in (B). Operons predicted to be activated have CenR binding sites upstream of the -35 promoter element, whereas operons predicted to be repressed have CenR binding sites that overlap either the TSS or the predicted promoter elements.