Trim41 is required to regulate chromosome axis protein dynamics and meiosis in male mice
Fig 2
Histological analysis of Trim41 KO male mice.
(A and B) Testis morphology (A) and testis/bodyweight of WT, Trim41 Het, Trim41 KO, and KO-Tg adult mice (B). Error bars indicate standard deviation. The numerical data are available in S3 Table (C) PAS staining of seminiferous tubules of adult mice. The seminiferous epithelium cycle was determined by germ cell position and nuclear morphology. Red arrows indicate elongated spermatids with abnormal head morphology. PLe: Preleptotene stage spermatocyte; ZySC: Zygotene stage spermatocyte; PaSC: Pachytene stage spermatocyte; DiSC: Diplotene stage spermatocyte; MeSC: metaphase spermatocyte; RST: round spermatid; EST: elongating/elongated spermatid. (D) TUNEL staining of seminiferous tubules of adult mice counterstained with hematoxylin. At least three male mice were analyzed. (E) The stacked bar graph of TUNEL positive cells. The seminiferous epithelial stages were roughly determined by the arrangement and nuclear morphology of the first layer of germ cells (spermatogonia and leptotene/zygotene stage spermatocytes). The numerical data are available in S3 Table (F) PAS staining of cauda epididymis of adult mice. At least three male mice were analyzed.