ER-PM membrane contact site regulation by yeast ORPs and membrane stress pathways
Fig 5
Effects of osh4-1ts oshΔ, Δ-s-tether, and osh4-1ts Δ-s-tether mutations on membrane stress pathways.
Heatmap analyses of transcriptional responses relative to WT (SEY6210) at 37°C for 1 h in osh4-1ts Δ-s-tether (CBY6031), Δ-s-tether (CBY5898), and osh4-1ts oshΔ (CBY926) cells affecting (A) UPR, (B) heat shock and (C) high-osmolarity glycerol (HOG) pathway genes. Downregulated genes are shown in red, upregulated genes are shown in blue. UPR-responsive genes were curated from Kimata et al. [86] and using the Saccharomyces Genome Database (SGD), heat shock genes were curated from Hsf1p target genes and HOG pathway genes compiled from listed target genes of Msn2p/4p, Smp2p, Hot1p, and Sko1p. Histograms within each column represent the number of transcripts for each color in the heatmap.