Histone demethylase IBM1-mediated meiocyte gene expression ensures meiotic chromosome synapsis and recombination
Fig 6
Immunofluorescence of IBM1 in the IBM1 trans-complemented plants at leptotene, zygotene, pachytene and diplotene.
Dual-immunofluorescence images with anti-FLAG antibody (magenta) and an antibody against the meiosis-specific cohesin protein SYN1 (green). An IBM1-FLAG tag fusion was expressed in ibm1-6 plants and IBM1 can be observed colocalizing with chromosomes (DAPI, blue) at leptotene, zygotene, pachytene but not diplotene. Scale bar: 5 μm.