Histone demethylase IBM1-mediated meiocyte gene expression ensures meiotic chromosome synapsis and recombination
Fig 4
IBM1 is required for meiotic recombination.
(A) Images showing FISH with centromere probe of WT, ibm1-4, ibm1-6, spo11-1, ibm1-6 spo11-1, msh4, ibm1-6 msh4, mus81, ibm1-6 mus81 metaphase I chromosome spreads. (B) and (C) Zygotene and pachytene localization of γ-H2AX in WT, ibm1-4 and ibm1-6 meiocytes. (D) Quantification of γ-H2AX foci at zygotene and pachytene in WT, ibm1-4 and ibm1-6 mutants. (E) and (F) showing zygotene and pachytene localization of γ-H2AX and DMC1 in WT, ibm1-4 and ibm1-6 meiocytes. (G) Quantification of DMC1 at zygotene and pachytene in WT, ibm1-4 and ibm1-6 mutants. Box-plots show the median and interquartile ranges and the whiskers extend to the maximum and minimum of number of foci. ** p-value<0.01 with two-tailed student t test. Scale bar: 5 μm.