Bracoviruses recruit host integrases for their integration into caterpillar’s genome
Fig 2
CvBV circles are disrupted in two distinct modes.
A: Schematic diagram of identifying the disruption sites of CvBV circles. Step I: DNA was extracted from hemocytes of caterpillar hosts 24 h p.p. The DNA used for sequencing consists of CvBV DNA, P. xylostella DNA and P. xylostella DNA with integrated CvBV DNA. Step II: Chimeric reads containing both nucleotides of CvBV and P. xylostella sequences were sorted out based on BLASTN. Step III: Chimeric reads were then mapped to CvBV genome to identify the fractured sites. Two modes of disruption were found for CvBV circles, which included Conservative-Broken Circles (CBCs) and Random-Broken Circles (RBCs). B: The ratios of a conserved disruption site for CBCs and RBCs during integration. The abscissa represents different circles of CvBV, and the ordinate represents the percentage of disruption at the same site.