Reduction of Derlin activity suppresses Notch-dependent tumours in the C. elegans germ line
Fig 6
Retrograde transport may contribute to cup-2 activity and cup-2 is required mainly within the germline to promote germ cell proliferation.
A. Quantification of phenotypic analysis of the effect of loss of snx-1, cup-2’s partner in retrograde transport, on suppression of puf-8(q725); glp-1(oz264) tumours. Phenotypes were analyzed by α-REC-8 and α-HIM-3 immunostaining. B. Quantification of phenotypic analysis of the effect of knockdown of cup-2 activity within the germline by using a rrf-1(pk1417) mutant background which largely restricts RNAi efficacy to the germline. cup-2 RNAi and gfp RNAi was performed by injection of dsRNA. puf-8(q725); glp-1(oz264) and cup-2(tm2838); puf-8(q725); glp-1(oz264) phenotypic analysis is included for reference. Phenotypes were analyzed by α-REC-8 and α-HIM-3 immunostaining.