The Arabidopsis PHD-finger protein EDM2 has multiple roles in balancing NLR immune receptor gene expression
Fig 3
EDM2 suppresses transcript levels of many NLRs and basal defense.
A. Distribution of all genes, defense genes, NLR genes and genes of several other functional categories (defined by enriched GO terms) that show significant transcript level changes in edm2-2 compared to WT. Besides defense genes and NLRs, only non defense-associated categories with at least 130 genes differentially expressed in edm2-2 compared to WT were considered. χ2 test of independence was performed to detect significant differences between actual and expected equal distribution (50% up-regulated and 50% down-regulated genes). ***: P-value <0.001. B. Transcript level changes of EDM2-affected NLR genes. Only NLR genes with significant transcript level differences between edm2-2 and WT are included. C. Defense phenotype of edm2 mutants infected with Pseudomonas syringae pv. tomato DC3000 (Pst DC3000). Bacterial multiplication was monitored at 3 hrs(0), 2 days post inoculation (dpi) and 3 dpi with a 2 ×108 cfu/mL bacterial suspension. Error bars represent SEM for three biological replicates. fls2 (SALK_141277) seedlings were used as a susceptible control. Statistical significance was determined by Student’s t-tests. *: P-value <0.05. **: P-value <0.01.