Factors enforcing the species boundary between the human pathogens Cryptococcus neoformans and Cryptococcus deneoformans
Fig 3
Unique phenotypes of C. neoformans x C. deneoformans hybrid progeny.
(A) Sexual reproduction structures, including hyphae, basidia, and basidiospores, produced by C. neoformans x C. deneoformans hybrid progeny derived from bilateral msh2Δ genetic crosses following dissection and micromanipulation onto YPD agar medium. Sexual structures were formed on YPD agar plates sealed with parafilm and incubated in light conditions at room temperature. From left to right, scale bars on microscopy images represent 100 μm, 50 μm, and 10 μm. (B) Frequency of hybrid progeny capable of producing hyphae on YPD agar medium, at room temperature, in light, sealed conditions. Error bars represent standard error of the mean. One-way ANOVA identified no statistically significant differences between the three groups.