Factors enforcing the species boundary between the human pathogens Cryptococcus neoformans and Cryptococcus deneoformans
Fig 2
Mating structure formation in C. neoformans x C. deneoformans genetic crosses and germination frequencies of hybrid progeny.
(A) C. neoformans x C. deneoformans genetic crosses produced robust hyphal filamentation after 6 days of incubation in dark conditions on MS medium. Scale bars in colony border images (left image of each set) represent 200 μm. Scale bars in basidia and basidiospore morphology images (right image of each set) represent 50 μm. (B) Average frequencies of basidia lacking basidiospores, or bald basidia, in C. neoformans x C. deneoformans genetic crosses. Error bars represent standard error of the mean. Statistical significance was determined with a one-way ANOVA and Tukey’s post hoc test. (C) Mean germination frequencies of C. neoformans x C. deneoformans hybrid progeny in wild-type, unilateral, and bilateral msh2Δ genetic crosses. Error bars represent standard error of the mean and statistical significance was determined by one-way ANOVA followed by Tukey’s post hoc test. *** indicates p<0.001.