Factors enforcing the species boundary between the human pathogens Cryptococcus neoformans and Cryptococcus deneoformans
Fig 1
Hypermutator phenotypes of JEC20a msh2Δ mutants.
(A) Fluctuation analysis on YPD + rapamycin + FK506 medium was performed to quantify the mutation rates (number of mutations per cell per generation) of four independent JEC20a msh2Δ mutants: msh2Δ-1, msh2Δ-2, msh2Δ-3, and msh2Δ-4. The JEC20a progenitor strain in which the msh2Δ mutants were constructed served as the negative control and an msh2Δ mutant in the KN99α genetic background served as the positive control. Points indicate mean mutation rates and error bars indicate 95% confidence intervals for the mean; 10 independent replicates of each strain were included in mutation rate calculation. (B) Spectra of mutations identified through sequencing of the FRR1 gene in rapamycin + FK506-resistant colonies from fluctuation analysis conducted in panel A. For JEC20a n = 9, for all other strains, n = 10.