Metabolic effects of skeletal muscle-specific deletion of beta-arrestin-1 and -2 in mice
Fig 3
HFD SKM-Barr2-KO mice show no metabolic phenotype in hyperinsulinemic-euglycemic clamp studies.
Hyperinsulinemic-euglycemic clamps were performed in conscious, unrestrained HFD control and SKM-Barr2-KO mice (adult males) that had been fasted for 5 h. (A) Time course of blood glucose levels and glucose infusion rates (GIR) during the course of clamps. (B) Body weights. (C) GIR during the steady-state period of the clamp. (D) Whole body glucose clearance (Rd). (E) Hepatic glucose production (hGP) under basal and steady-state conditions. (F) Glycolysis rate. (G) Glycogen storage rate. (H-P) Glucose uptake into SKM tissues (H-K) and other metabolically important tissues (L-P). Quad, quadriceps muscle; WAT, white adipose tissue; BAT, brown adipose tissue. 2-DG tissue uptake was determined as described in Materials and Methods. Data are shown as mean ± SEM (7–8 mice per group). Ns, no statistically significant difference (2-way-ANOVA).