Locus suicide recombination actively occurs on the functionally rearranged IgH allele in B-cells from inflamed human lymphoid tissues
Fig 1
3’RR structure and transcription in activated B-cells.
(A) Structure of genomic fragments including either human Sα1, Cα1 gene and 3’RR1 (left), or Sα2, Cα2 and 3’RR2 (right) was analyzed for presence of G4 DNA on the template (TS) or non-template (NTS) strand (top). Dot plot analysis of internal repeats (bottom) was run with the YASS algorithm: colored dots indicate short stretches of homology in direct (green) or inverted (red) orientation (green squares thus mark positions of highly repetitive S or LS regions / red lines indicate positions of inverted repeats, notably the palindrome centered on the hs1,2 enhancer). (B) Transcription of human 3’RR2 in sense and antisense orientation, as evaluated by RNA-seq experiments with RNA from in vitro activated B-cells.