ITPase deficiency causes a Martsolf-like syndrome with a lethal infantile dilated cardiomyopathy
Fig 5
Transcriptomic and proteomic analyses of Itpa null heart.
(A) Heatmap.2 based clustering of per-transcript genome wide log2 ratios from biological triplicates of RNAseq from Itpa-null embryonic hearts and littermate controls. (B,C) Plot of per-gene log2 signal from Affymetrix MTA1.0 microarray (same data as A) and quantitative protein mass spectrometry (same data as D) on samples from wild-type and Itpa-null embryonic hearts. (D) Heatmap.2 based clustering of genome wide per-protein intensity from six biological replicates of quantitative mass spectrometry from Itpa-null embryonic hearts and littermate controls. (E, F) Subset of data from A & D focussed on transcripts and proteins that are known to be involved in mendialian causes of dilated cardiomyopathy. No major differences are detectable in any of the heatmaps. (G) Quantitative RT-PCR (qPCR) of selected transcripts in Itpa-null embryonic hearts and littermate controls. The data shown are derived from analysis of 10 individual cDNA preparations per genotype, each analysed in triplicate.