Distinct regions of the intrinsically disordered protein MUT-16 mediate assembly of a small RNA amplification complex and promote phase separation of Mutator foci
Fig 5
The C-terminal region of MUT-16 is sufficient for foci formation.
(A) Diagram and amino acid coordinates of C-terminal regions of MUT-16 fused to GFP. (B) Western blot of full-length and C-terminal fragments of MUT-16::GFP probed with anti-GFP and anti-actin. A non-specific band (marked by asterisk) runs at a similar size to the full-length MUT-16::GFP. (C) Live imaging of full-length MUT-16::GFP, which forms foci throughout the germline. (D-G) C-terminal fragments of MUT-16::GFP form foci in the germline if they contain the minimal J, K, and L region (amino acids 773–1050) of the protein, but the number and size of foci increase with larger protein fragments. (H) The KL region (amino acids 885–1050) of MUT-16 fused to GFP is not sufficient for foci anywhere in the germline. All images are from the transition zone (leptotene/zygotene) region of the germline. Scale bars, 5μm.