A coupled process of same- and opposite-sex mating generates polyploidy and genetic diversity in Candida tropicalis
Fig 6
Progeny generated from “a x a +α helper” three-way matings showed diverse responses to amphotericin B and caspofungin.
The MIC value was determined in 74 progeny-derived strains with distinct colony appearances from the “a x a + α helper” mating. Diploid parental strains Ps1, Ps2, and Ps3 (CAY2060, GH1374h and CAY4149) served as controls. MIC assays were performed in 96-well plates. Different wells contained 200 μL of RPMI-1640 medium with amphotericin B or caspofungin in a series of concentrations (from 0.3 to 2.4 μg/mL). Three biological replicates were performed and the average values are presented. Error bars represents corresponding standard deviations.