Synergistic co-regulation and competition by a SOX9-GLI-FOXA phasic transcriptional network coordinate chondrocyte differentiation transitions
Fig 7
Competition between SOX9 and FOXA2 during chondrocyte differentiation.
(A-D) Competition between SOX9 and FOXA2 on Col2a1 intron 1 and Col10a1 enhancer by luciferase assay in ATDC5. Enh: enhancer; Pro: promoter. The amount of SOX9 was kept constant and FOXA2 was increased and vice versa. (E) EMSA was performed using probes for the Col2a1 intron I (Gel I and II) and Col10a1 enhancer (Gel III and IV), and SOX9 and FOXA proteins at indicated concentrations. The sequences from Col2a1 intron I and Col10a1 enhancer used in the EMSAs are shown in the upper panel, and the SOX9/FOXA binding motifs are indicated by capital letters. **: p-value < 0.01; *: p-value < 0.05; NS: Non-significant.