Drosophila DNA polymerase theta utilizes both helicase-like and polymerase domains during microhomology-mediated end joining and interstrand crosslink repair
Fig 1
Drosophila Pol θ ATPase activity is required for resistance to a DNA interstrand crosslinking agent but not ionizing radiation.
A. Schematic of mus308 alleles and transgenes. The N-terminal helicase-like domain (blue) and C-terminal polymerase domain (green) are connected by the central domain (gray). Control, ATPase-dead, and polymerase-dead (pol-dead) are transgenic alleles. Numbers indicate amino acid positions in the protein. B. Nitrogen mustard sensitivity survival curve for domain-specific mutants. Transgenes were present in a mus308Δ background. C. Ionizing radiation sensitivity survival curve for domain-specific transgenic mutants. All transgenes were in a mus308Δ, spn-A (rad51) background. Data points represent the average of three independent trials and standard deviations are shown. *p<0.05, **p<0.01, ***p<0.0001 compared to full-length control, two-way ANOVA, Tukey’s post-hoc test.